Microscopy unit

Microscopy for a long time has become a basic tool for many researchers. It allows us to solve structures that depending on the type of microscopy we use can range from a few nanometers to a few microns.

Microscopy unit of IDIVAL, currently has powerful optical microscopes (based on photons) and a transmission electron microscope (TEM) (based on electrons).

Within the optical microscopy we have a Nikon A1R confocal microscope and an Nikon Ti epifluorescence microscope designed to make time lapse. By the other hand, the transmission electron microscopy we have a JEOL_JEM 1011, equipment specially designed to work with biological samples due to its high contrast power.

Our Microscopy Unit offers a comprehensive service to researchers and provides technical and methodological support in the development of projects that require these techniques.

LOCATION AND PHONE:
IDIVAL building, Lab 011, floor 0    
Tel: +34 942 31 55 15 Ext.: 73219
Fax: +34 942 31 55 17

PERSONNEL:

Main Technician:

Dr. Fidel Madrazo Toca 
E-mail: microscopia2@idival.org

Laser Microscopy Unit Director
Dra. Mónica López Fanarraga 
E-mail: microscopia1@idival.org

Electronic Microscopy Unit Director
Dr. Miguel Angel Lafarga Coscojuela 
E-mail: miguel.lafarga@unican.es

Optical microscopy

  • Mono or multichannel confocal microscopy in tissue / fixed cell and living cell.
  • Mono or multichannel Epi-fluorescence in tissue / fixed cell and living cell.
  • Single-channel or multi-channel TIRF (Total Internal Reflexion) in tissue / fixed cell and living cell.
  • DIC for all Nikon A1R objectives.
  • Phase contrast for all Nikon Ti Epifluorescence Microscope objectives.
  • Spectral analysis (scanning in λ to study different emissions).
  • Resonant scanning for live confocal cell study at very high speed.
  • Studies in living cells in widefield, confocal or TIRF.
  • Obtaining images by transmission electron microscopy.
  • Specialized techniques and other services:
  • Time-lapse recording
  • Photo-bleaching (resonanate head) and simultaneous galvo scanning.
  • FRAP (Fluorescence Recovery After Photobleaching) techniques, FRET (Förster Resonance Energy Transfer) and FLIP (Fluorescence Loss In Photobleaching).
  • Simultaneous acquisition of 32-channel spectral images.

Electronic microscopy

  • Training and support in the management of equipment.
  • Calibration of the microscope before the sessions. 

Image Analysis

  • Analysis, treatment and quantification of images.
  • Cell tracking.
  • Deconvolution.

Normas de uso de los servicios de Microscopía

Normas de uso Videomicroscopía

Gobierno de Cantabria  Servicio Cántabro de Salud  Hospital Universitario Marqués de Valdecilla   Universidad de Cantabria  Campus Internacional  
INSTITUTO DE INVESTIGACIÓN MARQUÉS DE VALDECILLA
Edificio IDIVAL, Avenida Cardenal Herrera Oria s/n,
39011 Santander (CANTABRIA).

Phone.: 942 31 55 15
Fax: 942 31 55 17

 

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