The Flow Cytometry and Cell Sorting Unit is a platform that provides technical and methodological support in the use of flow cytometry and cell separation and in its different applications for the development of research projects that require the identification, characterization, quantification and / or separation of cells or particles through the use of specific fluorescent labels.
Yellow/green (561nm) Cell separation in tubes, plates and ports. Separation of sterile samples for subsequent culture. Separation of up to four populations simultaneously
CytExpert SRT™ Software
Magnetic Cell sorter AutoMACSTM (Miltenyi)
SERVICES
Processing and acquisition of cells for cytometric analysis.
Cell separation by FACSorting and/or combined with previous magnetic separation.
Cytometry analysis.
Technical support and advice to research groups that request it.
Basic and advanced training for users.
Design of cytometric analysis protocols.
Applications
Detection by fluorochrome-conjugated antibodies of surface or intracellular antigens in eukaryotes.
Cell viability, apoptosis and necrosis and cell cycle assays by dispersion and permeability measurements to vital dyes and DNA staining (Propidium Iodide, 7-AAD, annexin V).
Cell proliferation assays (CFSE).
Cell separation for culture and functional assays.
Quantification of soluble molecules by multiparametric analysis using microspheres and adapted to the flow cytometer (e.g., soluble cytokines).
